Mass Cytometry:Article Title: Age-induced alterations of granulopoiesis generate atypical neutrophils that aggravate stroke pathology.
Article Snippet: For mass cytometry: 89 Y anti-Human CD45 (clone HI30), 1:100, Fluidigm Cat#3089003; Purified anti-human CD45 (Maxpar® Ready) Antibody, 1:100, Biolegend Cat#304045; 141 Pr anti-Human CD49d (clone 9F10), 1:200, Fluidigm Cat3141004B; Nd anti-Human CD19 (clone HIB19), 1:200, Fluidigm Cat#3142001; Purified anti-Human CD11b activated (clone CBRM1/5), 1:200, Biolegend Cat#301402; Purified anti-Human CD47 (clone B6H12), 1:100 ,BD Cat#556044; 146 Nd anti-Human CD64 (clone FCGR1A / CD64), 1:100, Fluidigm Cat#3146006B; 147 Sm anti-Human CD11c (clone BU15), 1:300 Biolegend; Nd anti-Human CXCR2 (Monoclonal; clone 5E8/CXCR2), Biolegend; 148 Nd anti-Human CD34 (clone 581), 1:100, Fluidigm Cat#3148001B; Purified anti-Human CD35/CR1 (clone E11), 1:75 Biolegend Cat#333402; purified anti-Human CD41 (clone HIP8), 1:200, Biolegend; 151 Eu anti-Human CD123 (clone 6H6), 1:100, Fluidigm Cat#3151001B; 152 Sm anti-Human CD66b (clone G10F5), 1:100, Fluidigm Cat#3152011B; 153 Eu anti-Human CD101 (clone BB27) Biolegend; 154 Sm anti-Human CD3 (Monoclonal; clone UCHT-1), 1:200, Fluidigm Cat#3154003; Purified anti-Human CD177 (clone MEM-166), 4 nature portfolio | reporting sum m ary M arch 2021 1:100, Biolegend Cat#315802; Purified anti-Human PD-L1 (clone 29E.2A3), 1:200, Biolegend Cat#329715; 158 Gd anti-Human CD10 (clone HI10a), 1:200, Fluidigm Cat#3158011B; Purified anti-Human CD71 (clone CY1G4), 1:300, Biolegend Cat#334102; Purified antiHuman CD14 (clone M5E2), 1:200, Biolegend Cat#301801; Purified anti-Human CD15 (clone W6D3), 1:75, Biolegend Cat#323002; Purified anti-Human CD62L (clone FMC46), 1:100, Bio-Rad Cat#MCA1076G; 163 Dy anti-Human CD172a/b (clone SE5A5), 1:200, Fluidigm Cat#3163017B; Purified ICAM-1/CD54 (clone 14C11) Thermo Fisher Cat#MA5-23794; 165 Dy anti-Human CD16 (clone 3G8) Fluidigm; 166 Er anti-Human CD24 (clone ML5), 1:100, Fluidigm Cat#3166007B; 167 Er anti-Human CD38 (clone HIT2), 1:100, Fluidigm Cat#3167001B; 168 Er anti-Human Ki67 (clone B56), 1:200, BD Biosciences, 169 Tm anti-Human CD33 (clone WM53), 1:300, Fluidigm Cat#3169010B; Purified anti-Human CD117 (clone 104D2), 1:100, Biolegend Cat#313201; Purified anti-Human Siglec 8 (clone 7C9), 1:100, Biolegend Cat#347102; Purified anti-Human Basophils (clone 2D7), 1:200.
Purification:Article Title: Age-induced alterations of granulopoiesis generate atypical neutrophils that aggravate stroke pathology.
Article Snippet: For mass cytometry: 89 Y anti-Human CD45 (clone HI30), 1:100, Fluidigm Cat#3089003; Purified anti-human CD45 (Maxpar® Ready) Antibody, 1:100, Biolegend Cat#304045; 141 Pr anti-Human CD49d (clone 9F10), 1:200, Fluidigm Cat3141004B; Nd anti-Human CD19 (clone HIB19), 1:200, Fluidigm Cat#3142001; Purified anti-Human CD11b activated (clone CBRM1/5), 1:200, Biolegend Cat#301402; Purified anti-Human CD47 (clone B6H12), 1:100 ,BD Cat#556044; 146 Nd anti-Human CD64 (clone FCGR1A / CD64), 1:100, Fluidigm Cat#3146006B; 147 Sm anti-Human CD11c (clone BU15), 1:300 Biolegend; Nd anti-Human CXCR2 (Monoclonal; clone 5E8/CXCR2), Biolegend; 148 Nd anti-Human CD34 (clone 581), 1:100, Fluidigm Cat#3148001B; Purified anti-Human CD35/CR1 (clone E11), 1:75 Biolegend Cat#333402; purified anti-Human CD41 (clone HIP8), 1:200, Biolegend; 151 Eu anti-Human CD123 (clone 6H6), 1:100, Fluidigm Cat#3151001B; 152 Sm anti-Human CD66b (clone G10F5), 1:100, Fluidigm Cat#3152011B; 153 Eu anti-Human CD101 (clone BB27) Biolegend; 154 Sm anti-Human CD3 (Monoclonal; clone UCHT-1), 1:200, Fluidigm Cat#3154003; Purified anti-Human CD177 (clone MEM-166), 4 nature portfolio | reporting sum m ary M arch 2021 1:100, Biolegend Cat#315802; Purified anti-Human PD-L1 (clone 29E.2A3), 1:200, Biolegend Cat#329715; 158 Gd anti-Human CD10 (clone HI10a), 1:200, Fluidigm Cat#3158011B; Purified anti-Human CD71 (clone CY1G4), 1:300, Biolegend Cat#334102; Purified antiHuman CD14 (clone M5E2), 1:200, Biolegend Cat#301801; Purified anti-Human CD15 (clone W6D3), 1:75, Biolegend Cat#323002; Purified anti-Human CD62L (clone FMC46), 1:100, Bio-Rad Cat#MCA1076G; 163 Dy anti-Human CD172a/b (clone SE5A5), 1:200, Fluidigm Cat#3163017B; Purified ICAM-1/CD54 (clone 14C11) Thermo Fisher Cat#MA5-23794; 165 Dy anti-Human CD16 (clone 3G8) Fluidigm; 166 Er anti-Human CD24 (clone ML5), 1:100, Fluidigm Cat#3166007B; 167 Er anti-Human CD38 (clone HIT2), 1:100, Fluidigm Cat#3167001B; 168 Er anti-Human Ki67 (clone B56), 1:200, BD Biosciences, 169 Tm anti-Human CD33 (clone WM53), 1:300, Fluidigm Cat#3169010B; Purified anti-Human CD117 (clone 104D2), 1:100, Biolegend Cat#313201; Purified anti-Human Siglec 8 (clone 7C9), 1:100, Biolegend Cat#347102; Purified anti-Human Basophils (clone 2D7), 1:200.
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